Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System | Zendy

Yu Jiang | Zendy; Biao Chen | Zendy; Chunlan Duan | Zendy; Bingbing Sun | Zendy; Junjie Yang | Zendy; Sheng Yang | Zendy

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Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

Author(s) -

Yu Jiang,

Biao Chen,

Chunlan Duan,

Bingbing Sun,

Junjie Yang,

Sheng Yang

Publication year - 2015

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.04023-14

Subject(s) - genome editing , crispr , biology , cas9 , genome , escherichia coli , genetics , gene , computational biology , genome engineering , plasmid , streptococcus pyogenes , bacterial genome size , enterobacteriaceae , bacteria , staphylococcus aureus

An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to theEscherichia coli genome by using theStreptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with a highest efficiency of 100%, which was able to achieve simultaneous multigene editing of up to three targets. The system also demonstrated successful targeted chromosomal deletions inTatumella citrea , another species of theEnterobacteriaceae , with highest efficiency of 100%.

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