Characterization of a Novel Panton-Valentine Leukocidin (PVL)-Encoding Staphylococcal Phage and Its Naturally PVL-Lacking Variant | Zendy

Lynn El Haddad | Zendy; Sylvain Moineau | Zendy

Open Access

Characterization of a Novel Panton-Valentine Leukocidin (PVL)-Encoding Staphylococcal Phage and Its Naturally PVL-Lacking Variant

Author(s) -

Lynn El Haddad,

Sylvain Moineau

Publication year - 2013

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.03852-12

Subject(s) - leukocidin , panton–valentine leukocidin , lytic cycle , biology , staphylococcus aureus , prophage , virulence , gene , frameshift mutation , microbiology and biotechnology , genetics , integrase , open reading frame , genome , bacteriophage , virology , virus , exon , peptide sequence , bacteria , methicillin resistant staphylococcus aureus , escherichia coli

A new siphophage (LH1) was isolated from raw milk using aStaphylococcus aureus ST352 host. Its genome (46,048 bp, 57 open reading frames) includes the two genes encoding Panton-Valentine leukocidin (PVL), a virulence factor usually harbored byS. aureus prophages. Nine structural proteins were identified, including a tail protein generated through a +1 frameshift. A phage lytic mutant was isolated, and its analysis revealed the deletion of genes coding for the PVL and an integrase. The deletion likely occurred through recombination between direct repeats.

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