Behavior of Escherichia coli in a Heterogeneous Gelatin-Dextran Mixture
Author(s) -
Kathleen Boons,
Luc Mertens,
Eva Van Derlinden,
Charlotte C. David,
Johan Hofkens,
Jan Van Impe
Publication year - 2013
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.03782-12
Subject(s) - gelatin , dextran , microstructure , escherichia coli , confocal laser scanning microscopy , morphology (biology) , laser microscopy , confocal microscopy , confocal , enterobacteriaceae , biophysics , chemistry , chromatography , biology , biochemistry , crystallography , optics , microbiology and biotechnology , physics , gene , genetics
In a gelatin-dextran mixture, changing the (relative and/or absolute) concentration of the components leads to the formation of different microstructures. Confocal laser scanning microscopy illustrated that the nature of the microstructure determines the location and morphology of Escherichia coli colonies. Observations indicate that bacterial growth preferentially occurs in the dextran phase, regardless of the microstructure.
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