Direct Assessment of Metabolite Utilization by Pseudomonas aeruginosa during Growth on Artificial Sputum Medium | Zendy

Volker Behrends | Zendy; Benedikt Geier | Zendy; Huw D. Williams | Zendy; Jacob G. Bundy | Zendy

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Direct Assessment of Metabolite Utilization by Pseudomonas aeruginosa during Growth on Artificial Sputum Medium

Author(s) -

Volker Behrends,

Benedikt Geier,

Huw D. Williams,

Jacob G. Bundy

Publication year - 2013

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.03609-12

Subject(s) - metabolite , sputum , pseudomonas aeruginosa , metabolomics , excretion , microbiology and biotechnology , nuclear magnetic resonance spectroscopy , pseudomonas , extracellular , chemistry , biology , biochemistry , chromatography , bacteria , medicine , stereochemistry , tuberculosis , pathology , genetics

We grewPseudomonas aeruginosa in LB and artificial sputum medium (ASM) (filtered and unfiltered) and quantified metabolite utilization and excretion by nuclear magnetic resonance (NMR) spectroscopy (metabolic footprinting or extracellular metabolomics). Utilization rates were similar between media, but there were differences in excretion—e.g., acetate was produced only in unfiltered ASM.

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