Survival of Mycobacterium avium subsp. paratuberculosis in Synthetic Human Gastric Juice and Acidified Porcine Bile | Zendy

James Dalton | Zendy; Colin Hill | Zendy

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Survival of Mycobacterium avium subsp. paratuberculosis in Synthetic Human Gastric Juice and Acidified Porcine Bile

Author(s) -

James Dalton,

Colin Hill

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.03523-12

Subject(s) - paratuberculosis , propidium monoazide , mycobacterium avium subsp. paratuberculosis , microbiology and biotechnology , mycobacterium , biology , mycobacterium avium subspecies paratuberculosis , bacteria , polymerase chain reaction , biochemistry , gene , genetics

The bactericidal activities of synthetic gastric juice and acidified porcine bile on Mycobacterium avium subsp. paratuberculosis were assessed using propidium monoazide (PMA)-mediated quantitative reverse transcription-PCR, which allowed rapid relative quantitative analysis of viable M. avium subsp. paratuberculosis cells.

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