Open AccessDNA Probes for Unambiguous Identification of Listeria monocytogenes Epidemic Clone II Strains
Author(s) -
Ying Cheng,
J.-W. Kim,
Sangmi Lee,
Robin M. Siletzky,
Sophia Kathariou
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.03064-09
Subject(s) - listeria monocytogenes , outbreak , serotype , clone (java method) , biology , microbiology and biotechnology , listeria , virology , identification (biology) , dna , bacteria , genetics , ecology
Listeria monocytogenes epidemic clone II (ECII) strains have been responsible for two major multistate outbreaks of food-borne listeriosis in the United States, but their prevalence and ecology remain poorly understood. In this study, we describe DNA probes that unambiguously identify this clonal group. These probes were able to differentiate ECII strains of outbreak, sporadic, or environmental origin from otherL. monocytogenes strains of the same serotype (4b).