Use of a Fluorescent Analog of Glucose (2-NBDG) To Identify Uncultured Rumen Bacteria That Take Up Glucose | Zendy

Junyi Tao | Zendy; Courtney McCourt | Zendy; Halima Sultana | Zendy; Corwin D. Nelson | Zendy; John P. Driver | Zendy; Timothy J. Hackmann | Zendy

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Use of a Fluorescent Analog of Glucose (2-NBDG) To Identify Uncultured Rumen Bacteria That Take Up Glucose

Author(s) -

Junyi Tao,

Courtney McCourt,

Halima Sultana,

Corwin D. Nelson,

John P. Driver,

Timothy J. Hackmann

Publication year - 2019

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.03018-18

Subject(s) - bacteria , biology , rumen , biochemistry , fluorophore , stable isotope probing , agar , fluorescence , substrate (aquarium) , microbiology and biotechnology , microorganism , fermentation , genetics , ecology , physics , quantum mechanics

We introduce a method for identifying what substrates are consumed by bacteria in natural communities. Our method offers significant improvement over existing methods for studying this characteristic. Our method uses a fluorescently labeled substrate which clearly labels target bacteria (glucose consumers in our case). Previous methods use isotope-labeled substrates, which are notorious for off-target labeling (due to cross-feeding of labeled metabolites). Our method can be deployed with a variety of substrates and microbial communities. It represents a major advance in connecting bacteria to the substrates they take up.

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