Open AccessSelf-Formed Adaptor PCR: a Simple and Efficient Method for Chromosome Walking
Author(s) -
Shiming Wang,
Jian He,
Zhongli Cui,
Shunpeng Li
Publication year - 2007
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02973-06
Subject(s) - biology , chromosome , isolation (microbiology) , genome , computational biology , genetics , microbiology and biotechnology , bioinformatics , gene
We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes.
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