Open AccessShuttle Vector System for Methanococcus maripaludis with Improved Transformation Efficiency
Author(s) -
Alison D. Walters,
Sarah E. Smith,
J. Chong
Publication year - 2011
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02919-10
Subject(s) - shuttle vector , methanococcus , transformation (genetics) , genome , genetics , biology , vector (molecular biology) , computational biology , gene , recombinant dna , archaea
We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors inMethanococcus maripaludis . Strain S0001, containing ORF1 from pURB500 integrated into theM. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors.