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To investigate if the primary function of the Agr system of Listeria monocytogenes is to monitor cell density, we followed Agr expression in batch cultures, in which the autoinducer concentration was uniform, and in biofilms. Expression was heterogeneous, suggesting that the primary function of Agr is not to monitor population density.

Evidence of Autoinduction Heterogeneity via Expression of the Agr System of Listeria monocytogenes at the Single-Cell Level