Open AccessSearching for Mesophilic Thermotogales Bacteria: “Mesotogas” in the Wild
Author(s) -
Camilla Nesbø,
Rajkumari Kumaraswamy,
Marlena Dlutek,
W. Ford Doolittle,
Julia M. Foght
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02846-09
Subject(s) - bacteria , microbiology and biotechnology , mesophile , enterobacteriaceae , biology , escherichia coli , computational biology , genetics , gene
All cultivatedThermotogales are thermophiles or hyperthermophiles. However, optimized 16S rRNA primers successfully amplifiedThermotogales sequences from temperate hydrocarbon-impacted sites, mesothermic oil reservoirs, and enrichment cultures incubated at <46°C. We conclude that distinctThermotogales lineages commonly inhabit low-temperature environments but may be underreported, likely due to “universal” 16S rRNA gene primer bias.
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