Genetic Typing, Based on the 56-Kilodalton Type-Specific Antigen Gene, of Orientia tsutsugamushi Strains Isolated from Chiggers Collected from Wild-Caught Rodents in Taiwan | Zendy

Pey-Ru Lin | Zendy; Hui-Ping Tsai | Zendy; Pei-Yi Tsui | Zendy; Ming-Hui Weng | Zendy; MingDer Kuo | Zendy; Hung-Chi Lin | Zendy; Kuo-Ching Chen | Zendy; Dar-Der Ji | Zendy; Der-Ming Chu | Zendy; Wen-Tssann Liu | Zendy

Open Access

Genetic Typing, Based on the 56-Kilodalton Type-Specific Antigen Gene, of Orientia tsutsugamushi Strains Isolated from Chiggers Collected from Wild-Caught Rodents in Taiwan

Author(s) -

Pey-Ru Lin,

Hui-Ping Tsai,

Pei-Yi Tsui,

Ming-Hui Weng,

MingDer Kuo,

Hung-Chi Lin,

Kuo-Ching Chen,

Dar-Der Ji,

Der-Ming Chu,

Wen-Tssann Liu

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02796-10

Subject(s) - orientia tsutsugamushi , scrub typhus , biology , typing , virology , microbiology and biotechnology , rickettsiosis , genotype , gene , polymerase chain reaction , strain (injury) , sequence analysis , phylogenetic tree , genetics , rickettsia , virus , anatomy

Orientia tsutsugamushi is the etiological agent of scrub typhus, a mite-borne, febrile illness that occurs in the Asia-Pacific region. We conducted strain characterization ofO. tsutsugamushi isolates from chiggers obtained from rodents based the nucleotide sequence of the 56-kDa outer membrane protein gene. With the use of PCR, a total of 68 DNA sequences of 56-kDa antigen genes were amplified. Phylogenetic analysis revealed that there were at least six definable clusters among the 68 isolates: 37% Karp-related strains (25/68), 27% TA763 strains (18/68), 12% JG-related strains (8/68), 19% Kato-related strains (13/68), 4% divergent strains (3/68), and 1% representing a Gilliam prototype strain (1/68). Overall, theO. tsutsugamushi genotypes exhibited a high degree of diversity, similar to that seen in strains from the rest of the areas where scrub typhus is endemic. Moreover, the 56-kDa protein sequence similarity betweenO. tsutsugamushi isolates from mites and those from human patients (H. Y. Lu et al., Am. J. Trop. Med. Hyg. 83:658-663, 2010) were striking, thus highlighting potential risk factors for this emerging zoonotic disease.

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