Experimental Evolution of Escherichia coli K-12 in the Presence of Proton Motive Force (PMF) Uncoupler Carbonyl Cyanide m -Chlorophenylhydrazone Selects for Mutations Affecting PMF-Driven Drug Efflux Pumps | Zendy

Jessie M. Griffith | Zendy; Preston J. Basting | Zendy; Katarina M. Bischof | Zendy; Erintrude P. Wrona | Zendy; Karina S. Kunka | Zendy; Anna C. Tancredi | Zendy; Jeremy P. Moore | Zendy; Miriam Hyman | Zendy; Joan L. Slonczewski | Zendy

Open Access

Experimental Evolution of Escherichia coli K-12 in the Presence of Proton Motive Force (PMF) Uncoupler Carbonyl Cyanide m -Chlorophenylhydrazone Selects for Mutations Affecting PMF-Driven Drug Efflux Pumps

Author(s) -

Jessie M. Griffith,

Preston J. Basting,

Katarina M. Bischof,

Erintrude P. Wrona,

Karina S. Kunka,

Anna C. Tancredi,

Jeremy P. Moore,

Miriam Hyman,

Joan L. Slonczewski

Publication year - 2018

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02792-18

Subject(s) - efflux , tetracycline , escherichia coli , mutation , mutant , biology , microbiology and biotechnology , multiple drug resistance , point mutation , virulence , gene , genetics , drug resistance , antibiotics

The genetic responses of bacteria to depletion of proton motive force (PMF), and their effects on drug resistance, are poorly understood. PMF drives export of many antibiotics, but the energy cost may decrease fitness when antibiotics are absent. Our evolution experiment reveals genetic mechanisms of adaptation to the PMF uncoupler CCCP, including selection for increased CCCP efflux but also against the expression of PMF-driven pumps for drugs not present. The results have implications for our understanding of the gut microbiome, which experiences high levels of organic acids that decrease PMF.

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