Temperature- and Salinity-Decoupled Overproduction of Hydroxyectoine by Chromohalobacter salexigens | Zendy

Javier Rodríguez-Moya | Zendy; Montserrat Argandoña | Zendy; Fernando IglesiasGuerra | Zendy; Joaquı́n J. Nieto | Zendy; Carmen Vargas | Zendy

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Temperature- and Salinity-Decoupled Overproduction of Hydroxyectoine by Chromohalobacter salexigens

Author(s) -

Javier Rodríguez-Moya,

Montserrat Argandoña,

Fernando IglesiasGuerra,

Joaquı́n J. Nieto,

Carmen Vargas

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02774-12

Subject(s) - overproduction , plasmid , biology , salinity , gene , genetics , ecology

Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines.

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