English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Paratuberculosis is an infectious, chronic, and incurable disease that affects ruminants, caused byMycobacterium avium subsp.paratuberculosis . This bacterium is shed primarily through feces of infected cows but can be also excreted in colostrum and milk and might survive pasteurization. Since an association of genomic sequences ofM. avium subsp.paratuberculosis in patients with Crohn's disease has been described; it is of interest to rapidly detectM. avium subsp.paratuberculosis in milk for human consumption. IS900 insertion is used as a target for PCR amplification to identify the presence ofM. avium subsp.paratuberculosis in biological samples. Two target sequences were selected: IS1 (155 bp) and IS2 (94 bp). These fragments have a 100% identity among allM. avium subsp.paratuberculosis strains sequenced.M. avium subsp.paratuberculosis was specifically concentrated from milk samples by immunomagnetic separation prior to performing PCR. The amplicons were characterized using DNA methylase Genotyping, i.e., the amplicons were methylated with 6-methyl -adenine and digested with restriction enzymes to confirm their identity. The methylated amplicons from 100 CFU ofM. avium subsp.paratuberculosis can be visualized in a Western blot format using an anti-6-methyl -adenine monoclonal antibody. The use of DNA methyltransferase genotyping coupled to a scintillation proximity assay allows for the detection of up to 10 CFU ofM. avium subsp.paratuberculosis per ml of milk. This test is rapid and sensitive and allows for automation and thus multiple samples can be tested at the same time.

Rapid and Sensitive Method To Identify Mycobacterium avium subsp. paratuberculosis in Cow's Milk by DNA Methylase Genotyping