Quantification and Evaluation of Infectivity of Shiga Toxin-Encoding Bacteriophages in Beef and Salad | Zendy

Lejla Imamovic | Zendy; Maite Muniesa | Zendy

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Quantification and Evaluation of Infectivity of Shiga Toxin-Encoding Bacteriophages in Beef and Salad

Author(s) -

Lejla Imamovic,

Maite Muniesa

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02703-10

Subject(s) - shiga toxin , escherichia coli , infectivity , shigella , microbiology and biotechnology , biology , lysis , shiga like toxin , toxin , bacteria , polymerase chain reaction , enterobacteriaceae , virology , food science , virus , gene , genetics

Stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative PCR (qPCR). Stx phages from the samples were propagated in Escherichia coli C600, E. coli O157:H7, and Shigella strains and further quantified. Fifty percent of the samples carried infectious Stx phages that were isolated from plaques generated by lysis.

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