Open AccessDetermination of Glycosyltransferase Specificities for the Escherichia coli O111 O Antigen by a Generic Approach
Author(s) -
Gordon Stevenson,
Manuela Dieckelmann,
Peter R. Reeves
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02660-07
Subject(s) - escherichia coli , glycosyltransferase , gene , antigen , strain (injury) , enterobacteriaceae , biology , gene cluster , escherichia , computational biology , genetics , microbiology and biotechnology , anatomy
We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of theEscherichia coli O111 O-antigen GT genes.