Determination of Glycosyltransferase Specificities for the Escherichia coli O111 O Antigen by a Generic Approach | Zendy

Gordon Stevenson | Zendy; Manuela Dieckelmann | Zendy; Peter R. Reeves | Zendy

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Determination of Glycosyltransferase Specificities for the Escherichia coli O111 O Antigen by a Generic Approach

Author(s) -

Gordon Stevenson,

Manuela Dieckelmann,

Peter R. Reeves

Publication year - 2007

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02660-07

Subject(s) - escherichia coli , glycosyltransferase , gene , antigen , strain (injury) , enterobacteriaceae , biology , gene cluster , escherichia , computational biology , genetics , microbiology and biotechnology , anatomy

We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of theEscherichia coli O111 O-antigen GT genes.

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