Open AccessSolid-Phase Capture of Pathogenic Bacteria by Using Gangliosides and Detection with Real-Time PCR
Author(s) -
Prerak Desai,
Marie K. Walsh,
Bart C. Weimer
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02601-07
Subject(s) - bacteria , real time polymerase chain reaction , microbiology and biotechnology , biology , chromatography , pathogenic bacteria , sensitivity (control systems) , colony forming unit , chemistry , biochemistry , gene , genetics , electronic engineering , engineering
We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of ∼4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.