Open AccessReduction of Acid Tolerance by Tetracycline in Escherichia coli Expressing tetA (C) Is Reversed by Cations
Author(s) -
Kai F. Hung,
Jeffrey J. Byrd,
Jeffrey L. Bose,
Charles W. Kaspar
Publication year - 2006
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02519-05
Subject(s) - escherichia coli , rpos , magnesium , tetracycline , chemistry , sodium , potassium , calcium , sucrose , biochemistry , antibiotics , gene expression , gene , organic chemistry , promoter
When tetracycline was present,tetA (C) reduced acid tolerance, suppressedrpoS expression, and increased the concentration of total soluble proteins in stationary-phaseEscherichia coli . The suppression of acid tolerance was reversed by 85 mM sodium, potassium, magnesium, and calcium ions but not by 85 mM sucrose. Implications for using TetA(C) are discussed.