Differentiation of Bacillus anthracis , B. cereus , and B. thuringiensis by Using Pulsed-Field Gel Electrophoresis | Zendy

Wenwan Zhong | Zendy; Yulin Shou | Zendy; Thomas M. Yoshida | Zendy; Babetta L. Marrone | Zendy

Open Access

Differentiation of Bacillus anthracis , B. cereus , and B. thuringiensis by Using Pulsed-Field Gel Electrophoresis

Author(s) -

Wenwan Zhong,

Yulin Shou,

Thomas M. Yoshida,

Babetta L. Marrone

Publication year - 2007

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02478-06

Subject(s) - bacillus thuringiensis , bacillus anthracis , bacillus cereus , gel electrophoresis , bacillaceae , cereus , pulsed field gel electrophoresis , bacillales , electrophoresis , biology , microbiology and biotechnology , chromatography , chemistry , bacteria , genetics , gene , genotype , bacillus subtilis

A pulsed-field gel electrophoresis (PFGE) method was developed for discriminating Bacillus anthracis from B. cereus and B. thuringiensis. A worldwide collection of 25 B. anthracis isolates showed high-profile homology, and these isolates were unambiguously distinguished from B. cereus and B. thuringiensis isolates by cluster analysis of the whole-genome macrorestriction enzyme digestion patterns generated by NotI.

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