Listeria monocytogenes Biofilm-Associated Protein (BapL) May Contribute to Surface Attachment of L. monocytogenes but Is Absent from Many Field Isolates | Zendy

Suzanne J. Jordan | Zendy; Stefano Perni | Zendy; Sarah Glenn | Zendy; Isabel Fernandes | Zendy; Manuela Barbosa | Zendy; Manuela Sol | Zendy; Rogério Tenreiro | Zendy; Lélia Chambel | Zendy; Belarmino Barata | Zendy; Isabel Zilhão | Zendy; Timothy G. Aldsworth | Zendy; Andreia Adrião | Zendy; Maria Leonor Faleiro | Zendy; Gilbert Shama | Zendy; Peter W. Andrew | Zendy

Open Access

Listeria monocytogenes Biofilm-Associated Protein (BapL) May Contribute to Surface Attachment of L. monocytogenes but Is Absent from Many Field Isolates

Author(s) -

Suzanne J. Jordan,

Stefano Perni,

Sarah Glenn,

Isabel Fernandes,

Manuela Barbosa,

Manuela Sol,

Rogério Tenreiro,

Lélia Chambel,

Belarmino Barata,

Isabel Zilhão,

Timothy G. Aldsworth,

Andreia Adrião,

Maria Leonor Faleiro,

Gilbert Shama,

Peter W. Andrew

Publication year - 2008

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02419-07

Subject(s) - listeria monocytogenes , biofilm , virulence , microbiology and biotechnology , biology , in silico , foodborne pathogen , pathogen , surface protein , bacteria , abiotic component , escherichia coli , listeria , genetics , gene , virology , paleontology

Listeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utilized within the food industry, including stainless steel. The factors required for the attachment of this ubiquitous organism to abiotic surfaces are still relatively unknown. In silico analysis of the L. monocytogenes EGD genome identified a putative cell wall-anchored protein (Lmo0435 [BapL]), which had similarity to proteins involved in biofilm formation by staphylococci. An insertion mutation was constructed in L. monocytogenes to determine the influence of this protein on attachment to abiotic surfaces. The results show that the protein may contribute to the surface adherence of strains that possess BapL, but it is not an essential requirement for all L. monocytogenes strains. Several BapL-negative field isolates demonstrated an ability to adhere to abiotic surfaces equivalent to that of BapL-positive strains. BapL is not required for the virulence of L. monocytogenes in mice.

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