Cultivation of Fastidious Bacteria by Viability Staining and Micromanipulation in a Soil Substrate Membrane System | Zendy

Belinda C. Ferrari | Zendy; Michael R. Gillings | Zendy

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Cultivation of Fastidious Bacteria by Viability Staining and Micromanipulation in a Soil Substrate Membrane System

Author(s) -

Belinda C. Ferrari,

Michael R. Gillings

Publication year - 2009

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02407-08

Subject(s) - fastidious organism , bacteria , biology , staining , fluorescent staining , microorganism , membrane , microbiology and biotechnology , vital stain , soil bacteria , soil microbiology , biochemistry , genetics

Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

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