Transcriptome Analysis of an Antibiotic Downregulator Mutant and Synergistic Actinorhodin Stimulation via Disruption of a Precursor Flux Regulator in Streptomyces coelicolor | Zendy

Seon-Hye Kim | Zendy; Hanna Lee | Zendy; Hye-Jin Kim | Zendy; EungSoo Kim | Zendy

Open Access

Transcriptome Analysis of an Antibiotic Downregulator Mutant and Synergistic Actinorhodin Stimulation via Disruption of a Precursor Flux Regulator in Streptomyces coelicolor

Author(s) -

Seon-Hye Kim,

Hanna Lee,

Hye-Jin Kim,

EungSoo Kim

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02346-10

Subject(s) - streptomyces coelicolor , actinorhodin , transcriptome , mutant , biology , microarray analysis techniques , regulator , flux (metallurgy) , response regulator , streptomyces , regulon , microbiology and biotechnology , gene , genetics , chemistry , gene expression , bacteria , organic chemistry

Through microarray analysis of an antibiotic-downregulator-deleted Streptomyces coelicolor ΔwblA ΔSCO1712 mutant, 28 wblA- and SCO1712-dependent genes were identified and characterized. Among 14 wblA- and SCO1712-independent genes, a carbon flux regulating 6-phosphofructokinase SCO5426 was additionally disrupted in the ΔwblA ΔSCO1712 mutant and further stimulated actinorhodin production in S. coelicolor, implying that both regulatory and precursor flux pathways could be synergistically optimized for antibiotic production.

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