Open AccessChemotactic Behavior of Pathogenic and Nonpathogenic Leptospira Species
Author(s) -
Ambroise Lambert,
Naoko Takahashi,
Nyles W. Charon,
Mathieu Picardeau
Publication year - 2012
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02288-12
Subject(s) - leptospira , chemotaxis , microbiology and biotechnology , biology , biochemistry , chemistry , serotype , receptor
We have developed a capillary tube assay in combination with real-time PCR to quantitate the number of chemoattracted Leptospira cells. We identified Tween 80, glucose, sucrose, and pyruvate as attractants for Leptospira cells; amino acids and vitamin B(12) were found to be nonchemotactic or weakly chemotactic. This assay has the general applicability to further our understanding of leptospiral chemotaxis.
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