Open AccessRemoval of Free Extracellular DNA from Environmental Samples by Ethidium Monoazide and Propidium Monoazide
Author(s) -
Andreas Otto Wagner,
Cornelia Malin,
Brigitte A. Knapp,
Paul Illmer
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02288-07
Subject(s) - propidium monoazide , biology , microbiology and biotechnology , real time polymerase chain reaction , biochemistry , gene
Recently, new DNA extraction techniques (using ethidium monoazide and propidium monoazide) have been developed to discriminate between alive and dead bacterial cells. Nevertheless, for complex environmental samples, no data are available yet. In the present study, these new methods were applied to anaerobic-fermentor sludge and the results were compared to a conventional microbiological approach.