Open AccessDetection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by 15 N and 13 C Incorporation
Author(s) -
Erin M. Gallagher,
L. Y. Young,
L. McGuinness,
Lee J. Kerkhof
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02274-09
Subject(s) - trinitrotoluene , terminal restriction fragment length polymorphism , bacteria , anaerobic bacteria , anaerobic exercise , biology , microbiology and biotechnology , isotope , chemistry , nuclear chemistry , restriction fragment length polymorphism , biochemistry , gene , genetics , organic chemistry , physics , polymerase chain reaction , quantum mechanics , explosive material , physiology
2,4,6-Trinitrotoluene ((15)N or (13)C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [(15)N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [(13)C]TNT cultures and was related to Lysobacter taiwanensis.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom