A Derepression System Based on the Bacillus subtilis Sporulation Pathway Offers Dynamic Control of Heterologous Gene Expression | Zendy

Reindert Nijland | Zendy; JanWillem Veening | Zendy; Oscar P. Kuipers | Zendy

Open Access

A Derepression System Based on the Bacillus subtilis Sporulation Pathway Offers Dynamic Control of Heterologous Gene Expression

Author(s) -

Reindert Nijland,

JanWillem Veening,

Oscar P. Kuipers

Publication year - 2007

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02266-06

Subject(s) - derepression , bacillus subtilis , heterologous , biology , gene , genetics , heterologous expression , bacillaceae , gene expression , bacteria , recombinant dna , psychological repression

By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter.

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