Open AccessStrategy for In Situ Detection of Natural Transformation-Based Horizontal Gene Transfer Events
Author(s) -
Aurora Rizzi,
Alessandra Pontiroli,
Lorenzo Brusetti,
Sara Borin,
C. Sorlini,
A. Abruzzese,
Gian Attilio Sacchi,
Timothy M. Vogel,
Pascal Simonet,
Marco Bazzicalupo,
Kaare M. Nielsen,
Jean-Michel Monier,
Daniele Daffonchio
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02185-07
Subject(s) - transformation (genetics) , microscale chemistry , in situ , biology , horizontal gene transfer , gene transfer , fluorescence microscope , bacteria , fluorescence , gene , microbiology and biotechnology , computational biology , chemistry , genetics , optics , genome , mathematics education , mathematics , physics , organic chemistry
A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.