Open AccessUse of Green Fluorescent Protein To Monitor Cell Envelope Stress in Lactococcus lactis
Author(s) -
Ana B. Campelo,
Ana Rodrı́guez,
Beatriz Martı́nez
Publication year - 2009
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02177-09
Subject(s) - lactococcus lactis , green fluorescent protein , fluorescence , reporter gene , cell envelope , western blot , biology , microbiology and biotechnology , cell , chemistry , gene , bacteria , biochemistry , gene expression , genetics , physics , escherichia coli , lactic acid , quantum mechanics
A Lactococcus lactis reporter system suitable to detect cell envelope stress in high-throughput settings was developed by fusing the CesR-regulated promoter of llmg0169 to the gfp(uv) gene. A dot blot assay allowed fast detection of green fluorescent protein (GFP) fluorescence even at low production levels. Unexpectedly, this promoter was also induced by mitomycin C via CesR.
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