Cytosine Deaminase as a Negative Selection Marker for Gene Disruption and Replacement in the Genus Streptomyces and Other Actinobacteria | Zendy

Marie-Pierre Dubeau | Zendy; Mariana Gabriela Ghinet | Zendy; PierreÉtienne Jacques | Zendy; Nancy Clermont | Zendy; Carole Beaulieu | Zendy; Ryszard Brzeziński | Zendy

Open Access

Cytosine Deaminase as a Negative Selection Marker for Gene Disruption and Replacement in the Genus Streptomyces and Other Actinobacteria

Author(s) -

Marie-Pierre Dubeau,

Mariana Gabriela Ghinet,

PierreÉtienne Jacques,

Nancy Clermont,

Carole Beaulieu,

Ryszard Brzeziński

Publication year - 2008

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02139-08

Subject(s) - actinobacteria , biology , streptomyces , cytosine deaminase , gene , genetics , cytosine , escherichia coli , selection (genetic algorithm) , cytidine deaminase , bacteria , 16s ribosomal rna , genetic enhancement , computer science , artificial intelligence

We developed a novel negative selection system for actinobacteria based on cytosine deaminase (CodA). We constructed vectors that include a synthetic gene encoding the CodA protein fromEscherichia coli optimized for expression inStreptomyces species. Gene disruption and the introduction of an unmarked in-frame deletion were successfully achieved with these vectors.

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