Stability and Tick Transmission Phenotype of gfp -Transformed Anaplasma marginale through a Complete In Vivo Infection Cycle | Zendy

Susan M. Noh | Zendy; Massaro W. Ueti | Zendy; Guy H. Palmer | Zendy; Ulrike G. Munderloh | Zendy; Roderick F. Felsheim | Zendy; Kelly A. Brayton | Zendy

Open Access

Stability and Tick Transmission Phenotype of gfp -Transformed Anaplasma marginale through a Complete In Vivo Infection Cycle

Author(s) -

Susan M. Noh,

Massaro W. Ueti,

Guy H. Palmer,

Ulrike G. Munderloh,

Roderick F. Felsheim,

Kelly A. Brayton

Publication year - 2010

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02096-10

Subject(s) - biology , in vivo , anaplasmataceae , transmission (telecommunications) , green fluorescent protein , phenotype , tick , virology , anaplasmosis , anaplasma , microbiology and biotechnology , genetics , gene , electrical engineering , engineering

We tested the stability and tick transmission phenotype of transformedAnaplasma marginale through a completein vivo infection cycle. Similar to the wild type, thegfp -transformedA. marginale strain established infection in cattle, a natural reservoir host, and persisted in immune competent animals. The tick infection rates for the transformedA. marginale and the wild type were the same. However, there were significantly lower levels of the transformedA. marginale than of the wild type in the tick. Despite the lower levels of replication, ticks transmitted the transformant. Transformants can serve as valuable tools to dissect the molecular requirements of tick colonization and pathogen transmission.

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