Production of Glutaconic Acid in a RecombinantEscherichia coliStrain | Zendy

Ivana Djurdjević | Zendy; Oskar Zelder | Zendy; Wolfgang Buckel | Zendy

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Production of Glutaconic Acid in a RecombinantEscherichia coliStrain

Author(s) -

Ivana Djurdjević,

Oskar Zelder,

Wolfgang Buckel

Publication year - 2010

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.02049-10

Subject(s) - escherichia coli , fermentation , bacteria , strain (injury) , recombinant dna , yield (engineering) , biochemistry , enzyme , cofactor , anaerobic bacteria , biology , enterobacteriaceae , metabolic engineering , chemistry , microbiology and biotechnology , gene , genetics , anatomy , materials science , metallurgy

The assembly of six genes that encode enzymes from glutamate-fermenting bacteria converted Escherichia coli into a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via (R)-2-hydroxyglutarate, (R)-2-hydroxyglutaryl-coenzyme A (CoA), and (E)-glutaconyl-CoA to yield 2.7 ± 0.2 mM (E)-glutaconate in the medium.

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