Open AccessLongitudinal Study of Shiga Toxin-Producing Escherichia coli Shedding in Sheep Feces: Persistence of Specific Clones in Sheep Flocks
Author(s) -
Sergio Sánchez,
Remigio Martínez,
Alfredo García,
Jorge Blanco,
Jesús E. Blanco,
Miguel Blanco,
Ghizlane Dahbi,
Cecilia López,
Azucena Mora,
J. Rey,
J.M. Alonso
Publication year - 2009
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02043-08
Subject(s) - flock , feces , biology , persistence (discontinuity) , escherichia coli , colonization , microbiology and biotechnology , veterinary medicine , pulsed field gel electrophoresis , shiga toxin , shiga like toxin , genotype , ecology , genetics , medicine , geotechnical engineering , gene , engineering
To provide information on the persistence and maintenance of colonization with Shiga toxin-producingEscherichia coli (STEC) in sheep, pulsed-field gel electrophoresis analysis of STEC isolates (n = 145) belonging to serogroups O5, O91, and O146 from 39 healthy animals was performed in a 12-month longitudinal study carried out with four sheep flocks. At the flock level as well as the individual-animal level, the same clones were obtained on sampling occasions separated by as much as 11 months.