Open AccessInhibition of Species of the Aspergillus Section Nigri and Ochratoxin A Production in Grapes by Fusapyrone
Author(s) -
Mara Favilla,
Michelangelo Pascale,
Alessandra Ricelli,
Antonio Evidente,
Carmine Amalfitano,
Claudio Altomare
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01998-07
Subject(s) - ochratoxin a , aspergillus , biology , ochratoxins , section (typography) , botany , mycotoxin , advertising , business
Fusapyrone (FP), an antifungal natural compound, was tested against the three main ochratoxigenic species of theAspergillus sectionNigri . The MICs at 24 h were 6.0, 11.6, and 9.9 μg/ml forAspergillus carbonarius ,Aspergillus tubingensis , andAspergillus niger , respectively. Strong inhibition of growth and morphological changes were still observed at half the MIC after 7 days. The application of a 100 μg/ml FP solution in a laboratory assay on artificially inoculated grapes resulted in a significant reduction (up to 6 orders of magnitude) ofA. carbonarius CFU counts. Dramatic reductions of the ochratoxin A (OTA) content, compared to the content of the positive control (average amount of OTA, 112.5 ng/g of grape; three experiments), were obtained with the application of either 100 or 50 μg/ml of FP (0.6 or 5.1 ng/g of grape, respectively).