Open AccessGenomic Characterization and Transcriptional Studies of the Starch-Utilizing Strain Bifidobacterium adolescentis 22L
Author(s) -
Sabrina Duranti,
Francesca Turroni,
Gabriele Andrea Lugli,
Christian Milani,
Alice Viappiani,
Marta Mangifesta,
Laura Gioiosa,
Paola Palanza,
Douwe van Sinderen,
Marco Ventura
Publication year - 2014
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01993-14
Subject(s) - biology , pilus , bifidobacterium breve , transcriptome , glycome , extracellular matrix , starch , biochemistry , bifidobacterium , genetics , microbiology and biotechnology , gene , gene expression , bacteria , glycan , glycoprotein , lactobacillus , virulence
Bifidobacteria are members of the gut microbiota, but the genetic basis for their adaptation to the human gut is poorly understood. The analysis of the 2,203,222-bp genome ofBifidobacterium adolescentis 22L revealed a nutrient acquisition strategy that targets diet/plant-derived glycans, in particular starch and starch-like carbohydrates. Starch-like carbohydrates were shown to support the growth ofB. adolescentis 22L. Transcriptome profiling of 22L cultures grown underin vitro conditions or during colonization of the murine gut by RNA sequencing and quantitative real-time PCR assays revealed the expression of a set of chromosomal loci responsible for starch metabolism as well as for pilus production. Such extracellular structures include so-called sortase-dependent and type IVb pili, which may be involved in gut colonization of 22L through adhesion to extracellular matrix proteins.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom