Open AccessOptimization of RNA Extraction for PCR Quantification of Aromatic Compound Degradation Genes
Author(s) -
Weidong Kong,
Cindy H. Nakatsu
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01939-09
Subject(s) - degradation (telecommunications) , rna extraction , rna , gene , extraction (chemistry) , polymerase chain reaction , dna , biology , chemistry , chromatography , computational biology , microbiology and biotechnology , genetics , computer science , telecommunications
Seven different bacterial strains and primer sets and a mixed community were used to evaluate the use of reverse transcriptase quantitative PCR (Q-PCR) and Q-PCR of oxygenase genes to assess various approaches for monitoring the bioremediation of polluted sites. Differences in maximum activity were seen when different RNA extraction kits were compared.