Open AccessDetection and Quantification of Dehalogenimonas and “ Dehalococcoides ” Populations via PCR-Based Protocols Targeting 16S rRNA Genes
Author(s) -
Jun Yan,
Brian A. Rash,
Fred A. Rainey,
William M. Moe
Publication year - 2009
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01938-09
Subject(s) - dehalococcoides , biology , 16s ribosomal rna , ribosomal rna , genetics , gene , computational biology , homology (biology) , polymerase chain reaction , chemistry , organic chemistry , vinyl chloride , copolymer , polymer
Members of the haloalkane dechlorinating genusDehalogenimonas are distantly related to “Dehalococcoides ” but share high homology in some variable regions of their 16S rRNA gene sequences. In this study, primers and PCR protocols intended to uniquely targetDehalococcoides were reevaluated, and primers and PCR protocols intended to uniquely targetDehalogenimonas were developed and tested. Use of the genus-specific primers revealed the presence of both bacterial groups in groundwater at a Louisiana Superfund site.