Identification and Quantification of Methanogenic Archaea in Adult Chicken Ceca | Zendy

Suwat Saengkerdsub | Zendy; Robin C. Anderson | Zendy; Heather H. Wilkinson | Zendy; Woo-Kyun Kim | Zendy; David J. Nisbet | Zendy; Steven C. Ricke | Zendy

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Identification and Quantification of Methanogenic Archaea in Adult Chicken Ceca

Author(s) -

Suwat Saengkerdsub,

Robin C. Anderson,

Heather H. Wilkinson,

Woo-Kyun Kim,

David J. Nisbet,

Steven C. Ricke

Publication year - 2006

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01931-06

Subject(s) - methanogen , archaea , 16s ribosomal rna , biology , ribosomal rna , phylotype , methanosarcina , identification (biology) , bacteria , gene , microbiology and biotechnology , genetics , ecology

By using molecular methods for the identification and quantification of methanogenic archaea in adult chicken ceca, 16S rRNA genes of 11 different phylotypes, 10 of which were 99% similar toMethanobrevibacter woesei , were found. Methanogen populations, as assessed by cultivation, and the 16S rRNA copy number were between 6.38 and 8.23 cells/g (wet weight) and 5.50 and 7.19 log10 /g (wet weight), respectively.

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