Open AccessCritical Evaluation of the Volumetric “Bottle Effect” on Microbial Batch Growth
Author(s) -
Frederik Hammes,
Marius Vital,
Thomas Egli
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01914-09
Subject(s) - bottle , enumeration , bacterial growth , volume (thermodynamics) , chemistry , chromatography , biological system , environmental science , biology , mathematics , materials science , bacteria , thermodynamics , physics , combinatorics , composite material , genetics
We have analyzed the impact of surface-to-volume ratio on final bacterial concentrations after batch growth. We examined six bottle sizes (20 to 1,000 ml) using three independent enumeration methods to quantify growth. We found no evidence of a so-called volumetric bottle effect, thus contradicting numerous previous reports.