English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Using colony blot hybridization withstx 2 andeae probes and agglutination in anti-O157 lipopolysaccharide serum, we isolatedstx 2 -positive andeae -positive sorbitol-fermenting (SF) enterohemorrhagicEscherichia coli (EHEC) O157:NM (nonmotile) strains from initial stool specimens andstx -negative andeae -positive SFE. coli O157:NM strains from follow-up specimens (collected 3 to 8 days later) from three children. Thestx -negative isolates from each patient shared with the correspondingstx 2 -positive isolatesfliC H7 , non-stx virulence traits, and multilocus sequence types, which indicates that they arose from thestx 2 -positive strains by loss ofstx 2 during infection. Analysis of the integrity of theyecE gene, a possiblestx phage integration site in EHEC O157, in the consecutivestx 2 -positive andstx -negative isolates demonstrated thatyecE was occupied instx 2 -positive but intact instx -negative strains. It was possible to infect and lysogenize thestx -negativeE. coli O157 strains in vitro using anstx 2 -harboring bacteriophage from one of the SF EHEC O157:NM isolates. The acquisition of thestx 2 -containing phage resulted in the occupation ofyecE and production of biologically active Shiga toxin 2. We conclude that theyecE gene in SFE. coli O157:NM is a hot spot for excision and integration of Shiga toxin 2-encoding bacteriophages. SF EHEC O157:NM strains and theirstx -negative derivatives thus represent a highly dynamic system that can convert in both directions by the loss and gain ofstx 2 -harboring phages. The ability to recyclestx 2 , a critical virulence trait, makes SFE. coli O157:NM strains ephemeral EHEC that can exist asstx -negative variants during certain phases of their life cycle.

Recycling of Shiga Toxin 2 Genes in Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:NM