Transcriptional Regulation and Signal-Peptide-Dependent Secretion of Exolevanase (LsdB) in the Endophyte Gluconacetobacter diazotrophicus | Zendy

Carmen Menéndez | Zendy; Alexander BanguelaCastillo | Zendy; Jesús Caballero-Mellado | Zendy; Lázaro Hernández | Zendy

Open Access

Transcriptional Regulation and Signal-Peptide-Dependent Secretion of Exolevanase (LsdB) in the Endophyte Gluconacetobacter diazotrophicus

Author(s) -

Carmen Menéndez,

Alexander BanguelaCastillo,

Jesús Caballero-Mellado,

Lázaro Hernández

Publication year - 2009

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01887-08

Subject(s) - endophyte , secretion , microbiology and biotechnology , signal peptide , biology , peptide , botany , biochemistry , peptide sequence , gene

Gluconacetobacter diazotrophicus utilizes plant sucrose with a constitutively expressed levansucrase (LsdA), producing extracellular levan, which may be degraded under energetically unfavored conditions. Reverse transcriptase-PCR analysis revealed that lsdA and the downstream exolevanase gene (lsdB) form an operon. lsdB transcription was induced during growth with low fructose concentrations (0.44 to 33 mM) and repressed by glucose. Transport of LsdB to the periplasm involved N-terminal signal peptide cleavage. Type II secretion mutants failed to transfer LsdB across the outer membrane, impeding levan hydrolysis.

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