Open AccessGateway-Based Destination Vectors for Functional Analyses of Bacterial ORFeomes: Application to the Min System in Brucella abortus
Author(s) -
Régis Hallez,
JeanJacques Letesson,
Jean Vandenhaute,
Xavier De Bolle
Publication year - 2007
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01873-06
Subject(s) - gateway (web page) , brucella , computational biology , brucella abortus , vector (molecular biology) , biology , computer science , epitope , replication (statistics) , microbiology and biotechnology , virology , genetics , world wide web , brucellosis , antigen , gene , recombinant dna
Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.