Open AccessSurveillance of Listeria monocytogenes: Early Detection, Population Dynamics, and Quasimetagenomic Sequencing during Selective Enrichment
Author(s) -
Eva Wagner,
Annette Fagerlund,
Solveig Langsrud,
Trond Møretrø,
Merete Rusås Jensen,
Birgitte Moen
Publication year - 2021
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01774-21
Subject(s) - listeria monocytogenes , biology , nanopore sequencing , population , amplicon , metagenomics , dna sequencing , illumina dye sequencing , genetics , microbiology and biotechnology , computational biology , polymerase chain reaction , bacteria , gene , demography , sociology
We showed that a combination of a short primary enrichment combined with MDA and Nanopore sequencing can accelerate the traditional process of cultivation and identification ofL. monocytogenes . The use of Illumina MiSeq sequencing additionally allowed us to predict the presence of cooccurringL. monocytogenes strains.