Housefly (Musca domestica) and Blow Fly (Protophormia terraenovae) as Vectors of Bacteria Carrying Colistin Resistance Genes

Flies have the capacity to transfer pathogens between different environments, acting as one of the most important vectors of human diseases worldwide. In this study, we trapped flies on a university campus and tested them for mobile resistance genes against colistin, a last-resort antibiotic in human medicine for treating clinical infections caused by multidrug-resistant Gram-negative bacteria. Quantitative PCR assays we developed showed that 34.1% ofMusca domestica (86/252) and 51.1% ofProtophormia terraenovae (23/45) isolates were positive for themcr-1 gene, 1.2% ofM. domestica (3/252) and 2.2% ofP. terraenovae (2.2%, 1/45) isolates were positive formcr-2 , and 5.2% ofM. domestica (13/252) and 44.4% ofP. terraenovae (20/45) isolates were positive formcr-3 . Overall, 4.8% (9/189) of bacteria isolated from the flies were positive for themcr-1 gene (Escherichia coli : 8.3%, 4/48;Enterobacter cloacae : 12.5%, 1/8;Providencia alcalifaciens : 11.8%, 2/17;Providencia stuartii : 4.9%, 2/41), while none were positive formcr-2 andmcr-3 . Fourmcr-1 -positive isolates (twoP. stuartii and twoP. alcalifaciens ) from blow flies trapped near a dumpster had a MIC for colistin above 4 mg/ml. This study reportsmcr-1 carriage inProvidencia spp. and detection ofmcr-2 andmcr-3 after their initial identification in Belgium and China, respectively. This study suggests that flies might contribute significantly to the dissemination of bacteria, carrying these genes into a large variety of ecological niches. Further studies are warranted to explore the roles that flies might play in the spread of colistin resistance genes.IMPORTANCE Antimicrobial resistance is recognized as one of the most serious global threats to human health. An option for treatment of the Gram-negative ESKAPE (Enterococcus faecium ,Staphylococcus aureus ,Klebsiella pneumoniae ,Acinetobacter baumannii ,Pseudomonas aeruginosa , andEnterobacter species) bacteria with multiple drug resistance was the reintroduction of the older antibiotic colistin. However, a mobile colistin resistance gene (mcr-1 ) has recently been found to occur widely; very recently, two other colistin resistance genes (mcr-2 andmcr-3 ) have been identified in Belgium and China, respectively. In this study, we report the presence of colistin resistance genes in flies. This study also reports the carriage of colistin resistance genes in the genusProvidencia and detection ofmcr-2 andmcr-3 after their initial identification. This study will stimulate more in-depth studies to fully elucidate the transmission mechanisms of the colistin resistance genes and their interaction.