Open AccessEvaluation of Escherichia coli Host Strain CB390 for Simultaneous Detection of Somatic and F-Specific Coliphages
Author(s) -
Carolina Guzmán,
Laura Mocé-Llivina,
F. Lucena,
Juan Jofre
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01710-07
Subject(s) - escherichia coli , plasmid , somatic cell , strain (injury) , biology , microbiology and biotechnology , bacteriophage , enterobacteriaceae , host (biology) , genetics , gene , anatomy
Escherichia coli WG5, the strain recommended by the International Organization for Standardization (ISO) to detect somatic coliphages, was transformed to F+ by introducing the plasmid Famp, which rendered it capable of simultaneously detecting both somatic and F-specific coliphages. Indeed, this strain, CB390, proved as effective in detecting similar numbers of phages as the sum of somatic and F-specific bacteriophages detected by the host strains recommended by both the ISO and the U.S. Environmental Protection Agency standardized methods.