Microfluidic PCR Combined with Pyrosequencing for Identification of Allelic Variants with Phenotypic Associations among Targeted Salmonella Genes | Zendy

Min Yue | Zendy; Robert Schmieder | Zendy; Robert A. Edwards | Zendy; Shelley C. Rankin | Zendy; Dieter M. Schifferli | Zendy

Open Access

Microfluidic PCR Combined with Pyrosequencing for Identification of Allelic Variants with Phenotypic Associations among Targeted Salmonella Genes

Author(s) -

Min Yue,

Robert Schmieder,

Robert A. Edwards,

Shelley C. Rankin,

Dieter M. Schifferli

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01703-12

Subject(s) - pyrosequencing , salmonella , biology , allele , genetics , gene , identification (biology) , phenotype , polymerase chain reaction , computational biology , bacteria , botany

A novel targeted massive parallel sequencing approach identified genetic variation in eight known or predicted fimbrial adhesins for 46Salmonella strains. The results highlight associations between specific adhesin alleles, host species, and antimicrobial resistance. The differentiation of allelic variants has potential applications for diagnostic microbiology and epidemiological investigations.

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