Open AccessAnalysis of Dibenzothiophene Desulfurization in a Recombinant Pseudomonas putida Strain
Author(s) -
Javier Calzada,
Marı́a Teresa Zamarro,
Almudena Alcón,
Victoria E. Santos,
Eduardo Dı́az,
José Luis Garcı́a,
Félix Garcı́a-Ochoa
Publication year - 2009
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01682-08
Subject(s) - pseudomonas putida , dibenzothiophene , strain (injury) , recombinant dna , pseudomonadales , flue gas desulfurization , pseudomonadaceae , exponential growth , chemistry , pseudomonas , biochemistry , biology , bacteria , chromatography , microbiology and biotechnology , organic chemistry , enzyme , genetics , anatomy , mathematics , mathematical analysis , gene
Biodesulfurization was monitored in a recombinantPseudomonas putida CECT5279 strain. DszB desulfinase activity reached a sharp maximum at the early exponential phase, but it rapidly decreased at later growth phases. A model two-step resting-cell process combining sequentiallyP. putida cells from the late and early exponential growth phases was designed to significantly increase biodesulfurization.