Quantitative Detection of Perchlorate-Reducing Bacteria by Real-Time PCR Targeting the Perchlorate Reductase Gene | Zendy

Mamie NozawaInoue | Zendy; Mercy Jien | Zendy; Nicholas S. Hamilton | Zendy; Valley Stewart | Zendy; Kate M. Scow | Zendy; Krassimira R. Hristova | Zendy

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Quantitative Detection of Perchlorate-Reducing Bacteria by Real-Time PCR Targeting the Perchlorate Reductase Gene

Author(s) -

Mamie NozawaInoue,

Mercy Jien,

Nicholas S. Hamilton,

Valley Stewart,

Kate M. Scow,

Krassimira R. Hristova

Publication year - 2008

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01658-07

Subject(s) - perchlorate , bacteria , gene , biology , protein subunit , real time polymerase chain reaction , biochemistry , chemistry , microbiology and biotechnology , genetics , ion , organic chemistry

A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.

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