Open AccessQuantifying Oxidation of Cellulose-Associated Glucuronoxylan by Two Lytic Polysaccharide Monooxygenases from Neurospora crassa
Author(s) -
Olav A. Hegnar,
Heidi Østby,
Dejan M. Petrović,
Lisbeth Olsson,
Anikó Várnai,
Vincent G. H. Eijsink
Publication year - 2021
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01652-21
Subject(s) - xylan , cellulose , polysaccharide , neurospora crassa , biochemistry , cell wall , biology , microbiology and biotechnology , chemistry , mutant , gene
Plant cell wall polysaccharides are highly resilient to depolymerization by hydrolytic enzymes, partly due to cellulose chains being tightly packed in microfibrils that are covered by hemicelluloses. Lytic polysaccharide monooxygenases (LPMOs) seem well suited to attack these resilient copolymeric structures, but the occurrence and importance of hemicellulolytic activity among LPMOs remains unclear.