Open AccessConstruction of a Reporter Vector for the Analysis of Bifidobacterium longum Promoters
Author(s) -
Adrianne Klijn,
D Moine,
Michèle Delley,
Annick Mercenier,
Fabrizio Arigoni,
R. David Pridmore
Publication year - 2006
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01611-06
Subject(s) - bifidobacterium longum , plasmid , promoter , biology , escherichia coli , reporter gene , genetics , gene , computational biology , bifidobacterium , bacteria , gene expression , lactobacillus
In order to initiate studies on promoter activities inBifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on aB. longum cryptic plasmid and theEscherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using threeB. longum promoters.